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picture1_Analysis Ppt 66826 | Nephelometry Turbidimetry  Cls 332 Lab 4


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File: Analysis Ppt 66826 | Nephelometry Turbidimetry Cls 332 Lab 4
introduction turbid metric nephlometeric analysis when part of incident radiant energy is dissipated by absorption reflection and refraction while the remainder is transmit light as a function of the concentration ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 3 years ago
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  INTRODUCTION
  Turbid metric  + nephlometeric analysis:
      When part of incident radiant energy is 
   dissipated by absorption, reflection, and 
   refraction, while the remainder is transmit light 
   as a function of the concentration of the 
   dispersed phase is the basis of turbid metric 
   analysis  
                                    Nephlometer                                      Turbidimeter
    Definition                      the measurement of the intensity  of             Light passing through a medium 
                                    scattered light at right angles to the           with dispersed particles, so the 
                                    direction of the incident light as a function    intensity of light transmitted is 
                                    of the concentration of the dispersed            measured.
                                    phase ,It is most sensitive for very dilute 
                                    suspensions (100 mg/L).
    Instrument used                 Nephlometery machine                             spectrophotometer
    Type of light measured          Scattered light                                  Transmitted light
    Arrangement of photometer  measure the light scattered at right angle to         made in the same direction as 
                                    the direction of the propagation of light from  the propagation of the light from 
                                    the source.                                      the source.
                                    It could be movable detectors which allow         
                                    operator to vary the angle of detection
    Clinical uses                   Ag-Ab rxn, immunocomplex rxn,ppts,               Ag-Ab rxn, immunocomplex 
                                    lipoprotein                                      rxn,ppts, liver dis, protein in 
                                                                                     urine or CSF
  Nephelometry and Turbidimetry 
  INSTRUMENTATION:
  1- light source: 
  Tungsten its relatively low intensity makes it less useful 
   for samples with low light scattering. Alternatives are:
   quartz halogen lamp, xenon lamp and laser which have 
   higher intensities than tungsten lamp.
  2-lens assembly:
  Light enter the sample holder through lens assembly.
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...Introduction turbid metric nephlometeric analysis when part of incident radiant energy is dissipated by absorption reflection and refraction while the remainder transmit light as a function concentration dispersed phase basis nephlometer turbidimeter definition measurement intensity passing through medium scattered at right angles to with particles so direction transmitted measured it most sensitive for very dilute suspensions mg l instrument used nephlometery machine spectrophotometer type arrangement photometer measure angle made in same propagation from source could be movable detectors which allow operator vary detection clinical uses ag ab rxn immunocomplex ppts lipoprotein liver dis protein urine or csf nephelometry turbidimetry instrumentation tungsten its relatively low makes less useful samples scattering alternatives are quartz halogen lamp xenon laser have higher intensities than lens assembly enter sample holder...

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